Tattikota SG, Cho B, Liu Y, Hu Y, Barrera V, Steinbaugh MJ, Yoon S-H, Comjean A, Li F, Dervis F, Hung R-J, Nam J-W, Ho Sui S, Shim J, Perrimon N (2020) A single-cell survey of Drosophila blood. Ĭattenoz PB, Sakr R, Pavlidaki A, Delaporte C, Riba A, Molina N, Hariharan N, Mukherjee T, Giangrande A (2020, e104486) Temporal specificity and heterogeneity of Drosophila immune cells. Ĭho B, Yoon S-H, Lee D, Koranteng F, Tattikota SG, Cha N, Shin M, Do H, Hu Y, Oh SY, Lee D, Vipin Menon A, Moon SJ, Perrimon N, Nam J-W, Shim J (2020) Single-cell transcriptome maps of myeloid blood cell lineages in Drosophila. Guilliams M, Bonnardel J, Haest B, Vanderborght B, Wagner C, Remmerie A, Bujko A, Martens L, Thoné T, Browaeys R, De Ponti FF, Vanneste B, Zwicker C, Svedberg FR, Vanhalewyn T, Gonçalves A, Lippens S, Devriendt B, Cox E, Ferrero G, Wittamer V, Willaert A, Kaptein SJF, Neyts J, Dallmeier K, Geldhof P, Casaert S, Deplancke B, ten Dijke P, Hoorens A, Vanlander A, Berrevoet F, Van Nieuwenhove Y, Saeys Y, Saelens W, Van Vlierberghe H, Devisscher L, Scott CL (2022) Spatial proteogenomics reveals distinct and evolutionarily conserved hepatic macrophage niches. Masuda T, Sankowski R, Staszewski O, Böttcher C, Amann L, Sagar n, Scheiwe C, Nessler S, Kunz P, van Loo G, Coenen VA, Reinacher PC, Michel A, Sure U, Gold R, Grün D, Priller J, Stadelmann C, Prinz M (2019) Spatial and temporal heterogeneity of mouse and human microglia at single-cell resolution. Hedlund E, Deng Q (2018) Single-cell RNA sequencing: technical advancements and biological applications. Ĭox N, Crozet L, Holtman IR, Loyher P-L, Lazarov T, White JB, Mass E, Stanley ER, Elemento O, Glass CK, Geissmann F (2021) Diet-regulated production of PDGFcc by macrophages controls energy storage. Gold KS, Brückner K (2014) Drosophila as a model for the two myeloid blood cell systems in vertebrates. Holz A, Bossinger B, Strasser T, Janning W, Klapper R (2003) The two origins of hemocytes in Drosophila. Krejčová G, Danielová A, Nedbalová P, Kazek M, Strych L, Chawla G, Tennessen JM, Lieskovská J, Jindra M, Doležal T, Bajgar A (2019) Drosophila macrophages switch to aerobic glycolysis to mount effective antibacterial defense. Woodcock KJ, Kierdorf K, Pouchelon CA, Vivancos V, Dionne MS, Geissmann F (2015) Macrophage-derived upd3 cytokine causes impaired glucose homeostasis and reduced lifespan in Drosophila fed a lipid-rich diet. Péan CB, Schiebler M, Tan SWS, Sharrock JA, Kierdorf K, Brown KP, Maserumule MC, Menezes S, Pilátová M, Bronda K, Guermonprez P, Stramer BM, Andres Floto R, Dionne MS (2017) Regulation of phagocyte triglyceride by a STAT-ATG2 pathway controls mycobacterial infection. Kierdorf K, Hersperger F, Sharrock J, Vincent CM, Ustaoglu P, Dou J, Gyoergy A, Groß O, Siekhaus DE, Dionne MS (2020) Muscle function and homeostasis require cytokine inhibition of AKT activity in Drosophila. Sanchez Bosch P, Makhijani K, Herboso L, Gold KS, Baginsky R, Woodcock KJ, Alexander B, Kukar K, Corcoran S, Jacobs T, Ouyang D, Wong C, Ramond EJV, Rhiner C, Moreno E, Lemaitre B, Geissmann F, Brückner K (2019) Adult drosophila lack hematopoiesis but rely on a blood cell reservoir at the respiratory epithelia to relay infection signals to surrounding tissues. In this chapter, we give a detailed protocol to purify hemocyte nuclei from adult Drosophila, which can be used in subsequent analyses such as snRNA-seq. Yet, a specific protocol to isolate nuclei from adult hemocytes for snRNA-seq and study these cells in different experimental conditions was not available. Only recently, the Fly Cell Atlas provided a whole transcriptomic single-cell atlas via single-nuclei RNA-sequencing (snRNA-seq) of adult Drosophila including many different tissues and cell types where hemocytes were also included. These analyses revealed anatomical and functional Drosophila hemocyte subtypes dedicated to specific tasks. Embryonic and larval hemocytes in Drosophila have been recently analyzed in single-cell RNA-sequencing (scRNA-seq) approaches during infection and steady state. Technological advances in high-throughput sequencing approaches allowed to give an in-depth characterization of vertebrate macrophage populations and their heterogenous composition within different organs as well as changes in disease. Hence, Drosophila offers a powerful genetic toolbox to study macrophage function and genetically modulate these cells. Ontogeny and functions are largely conserved between vertebrate and invertebrate macrophages. In adult Drosophila, most of the hemocytes are macrophage-like cells (so called plasmatocytes), which serve various functions in organ homeostasis and immune defense.
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